The Plant Synthetic Biology Conference will take place in Barcelona, Spain on September 19-21, 2022. This Conference is jointly organised by the Centre for Research in Agricultural Genomics (CRAG, Barcelona, Spain) and the CEPLAS-Cluster of Excellence on Plant Sciences, University of Düsseldorf and Cologne (Germany). The meeting will bring some of the global leaders in the field and it will cover the most recent advances in plant synthetic biology, with an emphasis on enabling technologies for reprogramming plant cells, current agricultural and industrial applications, the development of new green models and several perspectives on the ethical and societal aspects of synthetic biology.